Biocare’s 4plus streptavidin-biotin detection systems are designed for
reliable, cost-effective, two-step detection, providing a high-level of
sensitivity. The vast majority of primary antibodies can be diluted two-fold
compared to other commercially available avidin-biotin detection systems.
Biocare’s prediluted antibodies are all standardized to work with the 4plus
detection system.4plus detection system can be used with mouse and rabbit
primary antibodies. After labeling the antigen with primary antibody, a
species-specific or universal, affinity-purified, biotinylated secondary
antibody is added to bind. Horseradish peroxidase (HRP) or alkaline phosphatase
(AP) labeled-streptavidin is then added. A chromogen/substrate is applied that
reacts with a HRP or AP to produce an intense color signal.
100mL of each, link and label