HMW & p63 CK cocktail 1,0 mL
Specificity and Comments:
The p53 homologue p63 encodes for different isotypes able to either
transactivate p53 reporter genes (TAp63) or act as p53-dominant-negatives. The
p63 is detected in prostate basal cells in normal prostate, however, is negative
in malignant tumors of the prostate gland. Thus p63 is useful as a differential
marker for benign and malignant tumors of prostate gland.
The high molecular weight (DE-SQ) recognizes cytokeratins 13, 14, 15, and
16. In normal prostate epithelia, DE-SQ positivity is confined to stratified
epithelia, myoepithelial cells and basal cells in the prostate gland and
bronchi. It stains virtually identical to 34Eß12. The combination of HMW CK and
p63 has been shown to be superior that each alone. The basal cell cocktail not
only increases the sensitivity of the basal cell detection, but also reduces the
staining variability and therefore renders the basal cell immunostaining more
consistent (see important reference on backside).
Species Reactivity: Human; others not tested.
Clone: DESQ + BC/1A4
Isotype: Mouse IgG1 and IgG2a
Known Applications: Immunohistochemistry (formalin–fixed, paraffin embedded
Positive Control: Prostatic intraepithelial neoplasia
HMW CK: Cytoplasmic p63: Nuclear
Buffer with protein carrier and preservative
Storage and Stability:
Store vial at 4°C. Stable for 18 months.